In analytical chemistry, a blanking reagent serves as a reference solution used to establish a baseline measurement in various analytical techniques like spectrophotometry or chromatography. It is composed of the same solvent or matrix as the sample being analyzed, but without the analyte of interest. This solution helps to account for background interferences or signals inherent in the analytical instrument or method, ensuring accurate measurements by subtracting out any contributions from impurities, contaminants, or solvent effects. Blank reagents are prepared meticulously using high-purity solvents and undergo filtration or purification steps to remove any potential sources of interference. They are measured using the same analytical instrument and conditions as the sample, providing a baseline or zero-point reference for comparison.